The "All-American" Twirling Line grew out of an original two-majorette team in 1939. Over the years, Purdue has become widely known for its twirling line; many of today’s twirlers are state and national champions in their own right. The music just wouldn't be complete without the high energy dazzle of these outstanding twirlers, who loft their gleaming batons to incredible heights. Long known as the "Cradle of Twirlers," Purdue has a reputation for featuring the "best of the best" through the school year.

The Silver Twins were added to the "All-American" Marching Band in 1960. Although the original Silver Twins were identical twin sisters, there have been many featured twirlers who were "look alike" twins. These young women always appear together in performances and photographs, and although the Silver Twins have each excelled in the past as individual twirlers, as a team they share the special bond of shared performance.

In 1962, another solo twirler position was created as a back-up for the Golden Girl. June Ciampa, who also served as Golden Girl V, was the first to fill this position. Dressed in Purdue's other color of black, she first performed as the International Twirler. This title was later replaced with the more obvious Girl in Black.

Purdue's multiple featured twirlers add variety to their shows.